Description
Pen-Strep is a routinely used antibiotic mix in mammalian cell cultures to prevent Bacterial contamination.
Composition: Contains 10000 units Penicillin and 10000 units Streptomycin in saline. Used at 1:100 concentration in cell culture media
Penicillin was originally purified from the fungi Penicillium and acts by interfering directly with the turnover of the bacterial cell wall and indirectly by triggering the release of enzymes that further alter the cell wall. Streptomycin was originally purified from Streptomyces griseus. It acts by binding to the 30S subunit of the bacterial ribosome, leading to inhibition of protein synthesis and death in susceptible bacteria. This solution contains 10,000 units/mL of penicillin and 10,000 µg/mL of streptomycin.
