Description
Mouse nkx3.1 is an androgen-regulated homeodomain gene with the expression of Nkx3.1 protein predominantly localized to the prostate epithelial cells(2). Anti-mouse Nkx3.1 antibody is the IgG fraction from rabbit anti-sera generated by immunizing the animals with purified recombinant mouse Nkx3.1 protein. To improve the specificity of the antibody, only the N-terminal domain of mouse Nkx3.1 was produced as immunogen. The IgG fraction was prepared by protein A affinity chromatography. IgG specific to mouse Nkx3.1 was further purified using an affinity matrix produced by cross-linking the immnunogen to agarose.
References
- Guan, B., Pungaltya, P. et al. 2008. Ubiquitination by TOPORS regulates the prostate tumor suppressor NKX3.1. J. Biol. Chem. 283(8):4834-4840.
- Bieberich, C. J., Fujita, K., He, W. W., and Jay, G. (1996). Prostate-specific and androgen-dependent expression of a novel homeobox gene. J Biol Chem 271, 31779-31782.
