Description
I27O™ is a unique recombinant polyprotein used for the calibration of and as a reference for force extension experiments using atomic force microscopes. It is supplied in a ready-to-use solution. The protein is composed of 8 repeats of the Ig 27 domain of human titin. Single molecule measurements give up to eight saw-tooth force-extension curves with the specifications below. The protein has durable elasticity that allows for repeated unfolding and refolding.
The graph below demonstrates the unfolding and refolding cycles of an I21RS8 protein probed with a double-pulse protocol. The protein is first stretched to count the number of domains that unfold, N-total, (a-c, top traces), and then it is relaxed to its initial length. A second extension after a delay, Δt, measures the number of refolded domains, N-refolded (a-c, bottom traces).
MSDS_I27O_AFM_Protein
TechBrief_I27OAFM_Print
