Description
Anti-human NKX3.1 is the IgG fraction from rabbit anti-sera generated by immunizing the animals with purified recombinant human NKX3.1 protein. The immunogen corresponds to the amino-terminal 153 amino acids of the human protein (1). The IgG fraction was prepared by Protein A affinity chromatography.
NKX3.1 is located on human chromosome 8p21.21. It is an androgen-regulated homeodomain gene with the expression of the NKX3.1 protein predominantly localized to prostate epithelial cells (2,3)
A soft tissue metastasis stained with H&E (A), NKX3.1 (B), PSA (C) and PSAP (D). Note the lack of staining with PSA, while NKX3.1 and PSAP still stain strongly. (Figure 4 from Gurel et al 2010. Am J Surg Pathol. 2010 August; 34(8): 1097–1105)
References
- Bethel, C. R. et al. 2006. Decreased NKX3.1 protein expression in focal prostatic atrophy, prostatic intraepithelial neoplasia and adenocarcinoma: association with Gleason score and chromosome 8p deletion. Cancer Res. 66:10683-10690.
- Abate-Shen, C., Shen, M. M. and Gelmann, E. 2008. Integrating differentiation and cancer: the Nkx3.1 homeobox gene in prostate organogenesis and carcinogenesis. Differentation; research in biological diversity. 76:717-727.
- Gurel, B. et al. 2010. NKX3.1 as a marker of prostatic origin in metastatic tumores. Am. J. Surg. Pathol. 34(8):1097-1105.
