Description
Turbo Broth™ is used for the cultivation of recombinant strains of E. coli and typically produces cell yields 4 to 5 times higher than that of LB Broth in shake flask cultures depending on the strain. They have higher plasmid copy numbers than LB, which can enhance expression. This media uses glycerol for a carbon source and has a rich nutrient base of amino acids, vitamins, inorganic minerals, and trace minerals. The media are buffered at pH 7.2 ± 0.2 with potassium phosphate to prevent a change in pH and provide a source of phosphate. Media is ideal for fermentation cultures. Biomass yields will depend on the process employed and on the strain utilized.
