Description
Power Prime-olate™ is proprietary media formulation developed by AthenaES™ for improved expression of recombinant proteins in E. coli. This media is composed of a complex, rich mixture of amino acids, vitamins, and a carbon source at higher levels than those of Terrific Broth. The media are buffered at pH 6.8 ± 0.2. Power Prime-olate™ is APF Certified™. For expression of recombinant proteins, induction should be done at cell densities three times that of LB Broth. Cell yields are typically 3 to 4 times that of LB Broth in shake flask cultures depending on the characteristics of the strain. This medium is suitable as the base for fermentation cultures. Biomass yields will depend on the process and the strain employed.
