Description
Power Prime Broth™, is an Animal product free media for improved expression of recombinant proteins in E. coli. This media is composed of a complex, rich mixture of amino acids, vitamins, and a carbon source at higher levels than those of Terrific Broth. The media is buffered at pH 6.8 ± 0.2. For expression of recombinant proteins, induction should be done at cell densities three times that of LB Broth. Cell yields are typically 3 to 4 times that of LB Broth in shake flask cultures depending on the characteristics of the strain. Several reports suggest that Power Broth™ can increase the amount of soluble protein accumulated for otherwise insoluble proteins. This medium is suitable as the base for fermentation cultures. Biomass yields will depend on the process and the strain employed.
