Description
Augmedium™ is a medium additive which conditions cells prior to induction of recombinant protein expression. This pre-induction conditioner increases the level of chaperone proteins which can improve the fraction of product that accumulates as soluble protein. Augmedium™ is specifically intended for use with strains in which the target protein accumulates as an insoluble aggregate. Augmedium™ is supplied as a powder for preparation of 50x concentrated stock solutions. It is available in 100g and 500g amounts.
The graph on the left shows the level of expression of LypA with increasing levels of Augmedium™. The amount of activity of LypA directly correlated with the concentration of Augmedium™ and showed a significant increase of LypA accumulation. IGP is the isogenic parent strain.
