Description
The SFM Screening Kit is intended for researchers seeking to identify the most appropriate SFM formulation for a specific cell type. The kit contains a 100mL sample of each of five serum-free media including: BRFF-BMZERO™, BRFF-EPM2™, BRFF-P4-8F™, DMEM/F12, and IMDM along with 25mL of FNC Coating Mix® for dramatically increasing cell attachment to the plastic growth substratum. Each of the media is complete, ready-to-use.
The cell photographs on below of a SFM Screening Kit experiment to determine the optimum serum-free media for the production of the prostate cancer cell line 267-B1. The cultures were seeded with 2 x 106 cells/mL into each of the 5 25cm2 tissue culture flasks pre-coated with FNC Coating Mix® and containing the following media: BMZERO™, DMEM/F12, EPM2™, P4-8F™ and IMDM. The cells were grown for 24 hours at 37°C at 5% CO2. The photographs (20x magnification) show the difference in cell growth between the different media. The cells in the BMZERO™ media yielded a more rapid monolayer development than the other media with the least growth in IMDM. These results demonstrated that BMZERO™ is the optimum medium for the growth of 267-B1 cells.
ApplicationManual_SFMKit v1-3
WhyChooseSFMTechBrief.
