Description
FNC Coating Mix® is a specially formulated reagent that dramatically increases the attachment of cells to the plastic substratum used for culturing mammalian cell lines. Increased cell attachment facilitates and encourages growth in adherent cell cultures. Many fastidious cell types such as human prostate and breast epithelial cells will attach and grow more effectively on FNC-coated plastic surfaces when cultured in the appropriate serum-free medium.
The photograph to left shows the difference in monolayer development with and without FNC Coating Mix®. BRFF-55T prostate cancer cells were grown in HPC1™ medium at the same seed concentration for 3 days at 37°C, 5% CO2. The flask shown on left was pre-coated with FNC Coating Mix™ whereas the flask on the right was not pre-treated. Through image analysis, there was approximately 85% greater monolayer development in the flask coated with FNC than in the non-treated flask.
